![]() ![]() So, this is the key difference between Klenow and T4 DNA polymerase. However, T4 DNA polymerase has high processivity and high proofreading activity than the Klenow fragment. Similar to the Klenow fragment, it contains both 5′→3′ polymerase and 3′→5′ exonuclease activities, and it lacks 5′→3′ exonuclease activity. On the other hand, T4 DNA polymerase is a polymerase coded by the bacteriophage T4. ![]() Therefore, Klenow fragment is a bacterial polymerase. It lacks 5′→3′ exonuclease activity of intact E. It has only 5′→3′ polymerase and 3′→5′ exonuclease activities. Klenow fragment is a large fragment of E. The below info-graphic lists more differences between Klenow and T4 DNA polymerase in tabular form. Thus, this is another difference between Klenow and T4 DNA polymerase. Moreover, T4 DNA polymerase has a strong proofreading activity than the Klenow fragment. T4 DNA polymerase has a high processivity than Klenow fragment. Klenow fragment is originated from a bacterium while T4 DNA polymerase is originated from a bacteriophage which is a virus. The key difference between Klenow and T4 DNA polymerase is the origin of each enzyme. What is the Difference Between Klenow and T4 DNA Polymerase? ![]()
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